Research
Transcription of eukaryotic protein-coding genes requires transfer of RNA polymerase II (Pol II) through nucleosomes. Nucleosomes are inherent barriers of transcription, and Pol II stalls at multiple locations within a nucleosome. Nucleosome core particle (NCP) consists of 145–147 base pairs of DNA wrapped around a histone protein octamer. Transcription elongation factors accompany Pol II to facilitate efficient transcription. They enable polymerase progression through NCPs and ensure re-establishment of chromatin after polymerase passage. The mechanisms underlying these processes, however, remain puzzling and poorly understood.
In our study we are revealing this long-standing open question by identifying elements of proteins that mediates interactions between Pol II and nucleosome.
CTD-code / CTD-some / RNA Pol II
We combine NMR, X-ray, SAXS, cryoEM, MD and MS to study the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAPII), which serves as a binding platform for many RNA/protein-binding factors involved in the regulation of the transcription cycle.
Chromatin RNA Pol II cross-talk
We study interaction between transcription elongation and mRNA processing factors and the C-terminal domain (CTD) of the RPB1 subunit of RNA polymerase II (RNAPII). Our aim is to unveil how these CTD-binders cross-talk to chromatin.
Transcription and repair
Using integrative structural approach, we aim at understanding the cross-talk between transcription an other key pathways that take place on DNA.